|
CABRI:DSMZ_MUTZ |
|
Accession_number |
ACC 588
|
|
Cell_line_name |
8709
|
|
Brief_description |
confirmed as mouse by species PCR established from the splenocytes of a
radiation-induced acute myeloid leukemia (AML) of the C3H inbred mouse strain in
1997
|
|
Description |
mouse acute myeloid leukemia established from the splenocytes of a radiation-
induced acute myeloid leukemia (AML) of the C3H inbred mouse strain in 1997
confirmed as mouse by species PCR
|
|
Depositor |
Dr. P. Finnon, NRPB, Oxon, UK
|
|
Morphology |
cells growing singly or in small clusters in suspension, partly slightly adherent
|
|
Culture_conditions |
80% RPMI 1640 + 20% FBS + 2 mM L-glutamine + 10 ng/ml muIL-3 or 10-20%
WEHI-3B supernatant split culture 1:2 twice a week; seed out at ca. 0.5 x 106
cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; after thawing it is advisable to culture
the cells first in 24-well plates and to add 10 ng/ml mIL-3 or 10-20% WEHI-3B
supernatant daily; viability may be low after thawing at 37C with 5% CO2 maximum
density at about 1.2 x 106 cells/ml
|
|
Viruses |
PCR: SMRV -
|
|
Properties |
|
|
Release_conditions |
-
|
|
Hazard |
-
|
|
Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 106 cells/ampoule
|
|
Doubling_time |
ca. 60-80 hours
|
|
Mycoplasma |
negative in microbiological culture, PCR assays
|
|
Bibliographic_reference |
Finnon et al., Int. J. Radiat. Biol., 77: 51 (2001) [PMID: 11747540
] |